FastPCR is a free software for Microsoft Windows and is based on a new approach in the design of PCR primers for standard and long PCRs, inverse PCR, direct amino acid sequence degenerate PCR, multiplex PCR, in silico PCR and group-specific PCR (common primers for multiple sequences), single primering PCR, automatically SSR loci detection and direct PCR primers design; for sequence alignments, clustering and any kind repeat sequence, MITE elements and SSR loci searching; restriction enzyme analysis.